Environment Management I

S OF PAPERS 201 four were analyzed for body composition. The analyses of gases were determined from d 19-21. The broilers were fed from d 14-21 three different grower diets containing 3000, 3100, and 3200 kcal ME/kg. The diets consisted primarily of corn, soybean meal and poultry fat. The 3100 and 3200 kcal ME diets contained increasing protein and amino acids to maintain equivalent nutrient density with the 3000 kcal diet. All statistical analysis were performed using JMP pro 11 (SAS institute, 2013). Multiple regression analysis was developed having 2 independent variables, ME and age, the dependent variables were various parameters of gas exchange. The parameters with statistical significance (p-value <0.05) were VO2 expressed in L/Kg 0.70 R2=0.83; and L/Kg FI R2=0.87; RER (Respiratory exchange ratio) R2 = 0.77. Thus, the conclusion of Trial 1 was that the system could estimate energy differences from VO2 and RER between the three diets tested. In Trial 2, four treatments were studied: control (3100 Kcal, 19 CP); control plus xylanase + β-glucanase; control plus protease; and control plus pectinase + β-glucanase. Materials and methods were similar to Trial 1 but in Trial 2 an Incomplete Randomized Design was performed with six replications per treatment. The ME was predicted from the equation VO2 L/Kg 0.70 for 21 day of age using the statistical tool Inverse Prediction for each treatment. The energy savings from the enzymes was calculated subtracting the ME from the control. All the dietary treatments with enzymes showed energy savings + 92kcal/ kg, +130kcal/kg, + 34kcal/kg, respectively, compared to the control. These two trials suggests that gas exchange using Indirect Calorimetry in an open circuit system may be powerful tool to account for ME from the diet and provide a consistent and faster method to give ME values for an enzyme matrix.